Figure 1

Construction and detection of FaeG–FedF–FanC–FasA–Fim41a fimbriae MEFA protein. A Schematic illustration of the constructed ETEC FaeG–FedF–FanC–FasA–Fim41a fimbriae MEFA protein. The K88 fimbriae major structural subunit (FaeG) and the F18 fimbriae minor subunit (FedF) were used as the backbone. Each of these backbone components had nucleotides coding for three surface-exposed—but less-antigenic—epitopes substituted for by nucleotides coding for most antigenic epitopes predicted from K99, 987P, and F41 fimbriae. This allowed for the final construction of the ETEC fimbriae MEFA protein. B Detection of purified FaeG–FedF–FanC–FasA–Fim41a MEFA protein by 12% SDS-PAGE and Coomassie blue staining. C The expressed FaeG–FedF–FanC–FasA–Fim41a MEFA protein was detected using anti-FaeG monoclonal antibody (1:1000), anti-FedF serum (1:4000), anti-FanC serum (1:4000), anti-FasA serum (1:4000), and anti-Fim41a serum (1:4000) along with an HRP-conjugated goat anti-mouse IgG (1:10,000).