Figure 4

The TM and tail domains of NSP2 contribute to aggresome autophagy. A 293T cells were transfected with empty vector, GFP-NSP2, and NSP2 truncated plasmids (NSP2-GFP, OTU-GFP, HV-GFP, TM-GFP and Tail-GFP). At 12 h, 24 h, and 36 h after transfection, cell lysates were harvested and used for western blot analyses with the indicated antibodies (anti-GAPDH, anti-GFP, anti-p62, and anti-LC3). B 293T cells were transfected with an empty vector, GFP-NSP2, and NSP2 truncated plasmid (NSP2-GFP, OTU-GFP, HV-GFP, TM-GFP, and Tail-GFP). The cells were then analyzed by confocal microscopy. GFP-tagged proteins are shown in green and endogenous LC3 proteins are shown in red. Yellow in the merged images represents the co-localization between proteins. Bar size: 25 µm.