Table 1 Genes studied in the present work.
Gene | Coding for | LPS phenotype | Comments | References |
|---|---|---|---|---|
wadB | Glycosyltransferase involved in the synthesis of Brucella LPS core lateral branch | S-LPS with core defect | B. abortus mutants shown to be attenuated in mice | |
wadD | Glycosyltransferase involved in the synthesis of Brucella LPS core lateral branch | S-LPS with core defect | B. abortus mutants shown to be attenuated in mice | [29] |
wbkF | Undecaprenyl-glycosyltransferase priming bactoprenol for O-chain polymerization | R-LPS with complete core | B. melitensis mutants shown to be attenuated in mice | [17] |
wzm | Permease of the ABC system translocating the O-chain to the periplasm | R-LPS with complete core | Build up cytoplasmic O-chain on the inner membrane. B. melitensis mutants shown to be attenuated in mice and to trigger antibodies to the O-chain | |
ppdK | Pyruvate phosphate dikinase (PEP ↔ pyruvate) | S-LPS | Required for growth on gluconeogenic media B. abortus mutants are attenuated in mice | |
pckA | Phosphoenolpyruvate carboxykinase (oxaloacetate → PEP) | S-LPS | Inactive in B. abortus but not in B. suis biovar 5. Together with ppdK is required for growth of B. suis biovar 5 in gluconeogenic media. B. suis biovar 5 mutated in both ppdK and pckA are attenuated in mice |