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Table 1 Genes studied in the present work.

From: Development of attenuated live vaccine candidates against swine brucellosis in a non-zoonotic B. suis biovar 2 background

Gene Coding for LPS phenotype Comments References
wadB Glycosyltransferase involved in the synthesis of Brucella LPS core lateral branch S-LPS with core defect B. abortus mutants shown to be attenuated in mice [30, 31]
wadD Glycosyltransferase involved in the synthesis of Brucella LPS core lateral branch S-LPS with core defect B. abortus mutants shown to be attenuated in mice [29]
wbkF Undecaprenyl-glycosyltransferase priming bactoprenol for O-chain polymerization R-LPS with complete core B. melitensis mutants shown to be attenuated in mice [17]
wzm Permease of the ABC system translocating the O-chain to the periplasm R-LPS with complete core Build up cytoplasmic O-chain on the inner membrane. B. melitensis mutants shown to be attenuated in mice and to trigger antibodies to the O-chain [17, 55]
ppdK Pyruvate phosphate dikinase (PEP ↔ pyruvate) S-LPS Required for growth on gluconeogenic media B. abortus mutants are attenuated in mice [33, 34]
pckA Phosphoenolpyruvate carboxykinase (oxaloacetate → PEP) S-LPS Inactive in B. abortus but not in B. suis biovar 5. Together with ppdK is required for growth of B. suis biovar 5 in gluconeogenic media. B. suis biovar 5 mutated in both ppdK and pckA are attenuated in mice [33, 34]