Figure 4

B. suisbv2 presents a SNP leading to a premature stop inpckA. The sites where premature stop codons occur are indicated with a vertical arrow. The SNP leading to premature stop in Bs2WT is indicated as D (for deletion). The functional pckA of B. microti and B. suis biovar 5, and the mutated pckA of B. abortus described previously are included as reference. In all brucellae, pckA is located upstream of the regulatory gene bvrR. In Brucella presenting an intact pckA, yaeJ (coding a ribosome-associated protein) is located immediately downstream of pckA, whereas in the sequenced Brucella presenting a mutated pckA a ORF (i.e.: a truncated PckA in B. abortus or a pseudogene in B. suis bv2 Thomsen) upstream of yaeJ is annotated (stripped arrows).