Figure 4

Capillary electrophoresis plots illustrating invasive disease-associated and non-disease-associated SwissS. suisisolates. On the left hand side the DNA size marker (100 bp–2.5 kb) is shown. The alignment marker (green) is representing the start and end of electrophoresis. Amplicons sizes obtained from the molecular pathotyping tool [31] are indicated on the right hand side. Lane 1 corresponds to the amplification reaction of a highly virulent isolate of cps type 2 (sequence type 1103) revealing both disease-associated markers (red) predicting a putative copper exporting ATPase 1 (SSU0207) and a type I restriction-modification (RM) system S protein (SSU1589). Lane 2 and 3 represent disease-associated isolates harboring the copper ATPase and a variant form with a 21 base pair (bp) deletion (lane 3), respectively. Lane 4, 5, and 6 demonstrate observed amplification patterns of non-disease-associated samples (blue). Lane 4 shows a PCR amplicon for the putative sugar ABC reporter (SSUST30534) also in the presence of the copper ATPase gene. Samples with no amplification of any markers are considered to be non-disease-associated (lane 5). A sporulation regulator (WhiA) serves as identification control for S. suis (SSU0577).