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Figure 5 | Veterinary Research

Figure 5

From: Intercellular transmission of Seneca Valley virus mediated by exosomes

Figure 5

Exosome-mediated SVV infection is not blocked by SVV-specific neutralizing antibodies (NAbs). Purified SVV-positive exosomes and SVV were diluted and then the diluted exosomes and SVV were incubated with SVV-specific NAbs separately (the titer of serum neutralization against SVV was > 1:1024, determined by VNT) for 1 h. Exosomes and SVV without any treatment were used as a control. Then, IBRS-2 cells were exposed to the NAbs-treated exosomes or SVV for 2 h. The exosomes or viruses were washed off with PBS at 37 °C, and the medium was replaced with fresh maintenance medium for 24 h. RT-qPCR was used to evaluate SVV replication in IBRS-2 cells.

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