Figure 4

Exosomes promote the proliferation of SVV in IBRS-2. Exosomes were extracted from SVV-infected IBRS-2 cells and normal IBRS-2 cells separately, and the A280/A260 method was used to determine the protein concentration of SVV-exo and mock-exo. SVV-exo and mock-exo were treated with RNase for 1 h at 37 °C before they were incubated into IBRS-2 cells. Different doses of SVV-exo and mock-exo was incubated into IBRS-2 cells, SVV with the same SVV copy number as SVV-exo infected IBRS-2 cells and mock-exo, normal IBRS-2 cells as control, 8 h after incubation, the same copy number of SVV were infected again in the SVV-exo experimental group, mock-exo and SVV control groups, respectively. SVV copy number was detected using RT-qPCR at 24 h and 48 h after SVV infection. A The exosome protein concentration was inoculated at a dose of 25 ng, and the viral load was measured 24 h after SVV infection. B The exosome protein concentration was inoculated at a dose of 25 ng, and the viral load was measured 48 h after SVV infection. C The exosome protein concentration was inoculated at a dose of 50 ng, and the viral load was measured 24 h after SVV infection. D The exosome protein concentration was inoculated at a dose of 25 ng, and the viral load was measured 48 h after SVV infection. This experiment was repeated three times, all data represent mean ± SD, n = 3 for each group. Significant difference was calculated using two-tailed t-test and labeled as *P < 0.05 and **P < 0.01 in graphs.