Figure 2

Exosomes mediate the spread of SVV in susceptible and non-susceptible cells. A SVV VP1 gene was identified in SVV-exo by the RT-qPCR, at the same time, SVV, SVV-infected IBRS-2 cells (Cell-svv) was used positive control, normal IBRS-2 cells (Cell-mock), and mock-exo were used as negative controls. B SVV polyclonal antibody used to detect SVV protein levels in SVV-exo, also use cell-svv as a control. C SVV-exo were incubated with 293T and IBRS-2 cells, and at the same time, mock-exo, SVV with the same viral load as SVV-exo, and PBS were used as controls. The virus copy number was detected by RT-qPCR 24 h after incubation. D Exosomes extracted from SVV-GFP-infected cells (SVV-GFP-exo) were stained with DIL, washed twice by ultracentrifugation (Avoid light during this experiment), and incubated in 293T and IBRS-2 cells; same procedures were followed for SVV-GFP as a control. Eight hours after incubation, the nuclei were stained with DAPI, and fluorescence was observed.