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Table 3 Virus detection via RRT-PCR and the hemagglutinin type obtained directly from nasal swabs and bronchoalveolar lavage fluid (BALF) from pigs after contact with infected seeders and at necropsy by treatment group.

From: Exploring heterologous prime-boost vaccination approaches to enhance influenza control in pigs

Sample type

Nasal swabs (by day)

BALF

Treatment group

2 dpc

4 dpc

5 dpc

6 dpc

At necropsy (7 dpc)

PRa

Ct valueb

H1d

H3

PR

Ct value

H1

H3

PR

Ct value

H1

H3

PR

Ct value

H1

H3

PR

Ct value

H1

H3

COM/COM

1/10

43.54 (4.61)c A

0/10

0/10

2/10

41.95 (6.67)A

0/10

1/10

5/10

39.06 (7.41)A

0/10

1/10

3/10

40.26 (8.50)A

0/10

1/10

6/10

32.90 (11.70)BC

1/10

4/10

AUT/AUT

1/10

43.66 (2.85)A

0/10

0/10

5/10

39.84 (5.67)A

1/10

0/10

5/10

38.90 (7.21)A

1/10

0/10

1/10

43.05 (6.16)A

1/10

0/10

3/10

40.74 (6.38)A

1/10

1/10

AUT/COM

0/10

44.39 (1.84)A

0/10

0/10

7/10

38.12 (5.02)AB

0/10

0/10

5/10

40.01 (5.41)A

0/10

1/10

0/10

44.35 (2.06)A

0/10

0/10

1/10

43.93 (3.38)A

0/10

0/10

COM/AUT

1/10

43.95 (3.33)A

0/10

0/10

5/10

39.67 (5.74)A

0/10

0/10

5/10

39.71 (5.76)A

0/10

1/10

2/10

42.18 (6.31)A

0/10

1/10

4/10

39.89 (6.76)AB

0/10

2/10

NO VAC/CHA

5/10

37.83 (8.02)B

1/10

3/10

6/10

33.05 (9.71)B

3/10

4/10

7/10

30.43 (12.09)B

3/10

4/10

6/10

30.72 (10.89)B

1/10

4/10

8/10

26.75 (10.75)C

7/10

4/10

NO VAC/NO CHA

0/3

45 (0)

0/3

0/3

0/3

45 (0)

0/3

0/3

0/3

45 (0)

0/3

0/3

0/3

45 (0)

0/3

0/3

0/3

45 (0)

0/3

0/3

LAIV/COM

0/10

44.25 (1.83)A

0/10

0/10

5/10

39.33 (6.51)A

0/10

1/10

5/10

39.58 (6.09)A

0/10

1/10

3/10

40.20 (7.76)A

1/10

2/10

5/10

36.29 (9.66)A

1/10

4/10

LAIV/NONE

7/10

36.04 (6.70)B

1/10

3/10

6/10

32.04 (11.36)B

2/10

4/10

8/10

29.56 (9.95)B

3/10

4/10

7/10

32.26 (8.23)B

2/10

4/10

10/10

23.93 (5.24)B

7/10

6/10

  1. dpc: days post-contact; BALF: bronchoalveolar lavage fluid.
  2. a Positive rates (PR) are presented as the number of pigs with influenza A virus matrix gene RRT-PCR positive nasal swabs or BALF (numerator) by day post-contact (or at necropsy) out of the total number of pigs (denominator) in different treatment groups. The positive cutoff cycle threshold (Ct) value is 38.
  3. b Ct values are presented as mean (standard deviation). The actual Cts are used for samples with Ct values between 38 to 45 and the samples with Ct values above 45 are considered as 45 in the analysis.
  4. c Ct values with different superscripts (A, B, C) are significantly different (P < 0.05).
  5. d Number of pigs with H1 and H3 hemagglutinins detected by Next Generation Sequencing by treatment group at 2, 4, 5, and 6 days post-contact and at necropsy. Results are shown as number of positive pigs with H1 or H3 sequences/total number of pigs in the treatment group.