Figure 3

Virus shedding in upper and lower respiratory tract from pigs by treatment group after challenge. A Influenza A virus titer in bronchoalveolar lavage fluid (BALF) supernatant samples from vaccinated pigs at necropsy (7 days post-contact) by treatment group. B Influenza A virus titer of nasal swab samples collected at 2, 4 and 6 days post-contact by treatment groups. The virus titers are presented as log₁₀ TCID₅₀/mL and the values are shown as mean (bar) + SD (error line). The number of pigs shedding IAV is shown above each bar as a ratio of IAV positive pigs/total number of pigs tested. Pigs with virus titer above the detection limit of 1.75 TCID₅₀/mL (detection limit shown as dashed line) were considered virus positive pigs. The asterisks denote the significant difference (P < 0.05) in virus titer between groups calculated using a generalized linear mixed model (GLM) and Tukey was used for multiple LSmeans comparisons. The dark blue, green and dark red bars and data points represent the pigs from the whole inactivate vaccine comparison, negative control and live attenuate vaccine comparison groups, respectively.