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Figure  3 | Veterinary Research

Figure  3

From: EtMIC3 and its receptors BAG1 and ENDOUL are essential for site-specific invasion of Eimeria tenella in chickens

Figure  3

Binding of rEtMIC3, rEtMIC2, rEtAMA1 and rEtMIC1 to upper, mid, lower intestine and caecum of chicken intestines. Histological sections from upper, mid, lower intestine and caecum of chickens were incubated with rEtMIC3, rEtMIC2 rEtAMA1 and rEtMIC1 separately and then incubated with the corresponding antisera of the recombinant proteins separately. The vector protein control was treated with pET-32a vector protein and incubated with its antiserum. The negative control was treated with sterile PBS and incubated with serum from unimmunized rat. The binding ability was observed by immunofluorescence assay, and obvious red fluorescence was considered as positive. A rEtMIC3 specifically bound to caecum, but not other parts of the intestine of chickens. rEtMIC2 (B) rEtAMA1 (C) and rEtMIC1 (D) did not bind to any part of chicken intestine. (E, F, G) pET-32a vector protein (vector protein control) did not bind to any part of chicken intestine. H No obvious red fluorescence was observed in negative control.

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