Figure  2
Validation of the rEtMICs antisera by recognizing native EtMICs proteins fromE. tenellasporozoites. Lysate of E. tenella sporozoites was analyzed by western blot using antisera of rEtMIC3, rEtMIC2, rEtAMA1 and rEtMIC1 as primary antibody separately. Antisera of rEtMIC3 (lane 1), rEtMIC2 (lane 2), rEtAMA1 (lane 3) and rEtMIC1 (lane 4) recognized the corresponding native proteins from the lysate of E. tenella sporozoites respectively. The negative serum (lane 5) and pET-32a tag protein antiserum (lane 6) did not reacted with any native protein of E. tenella sporozoites. Lane M, standard protein molecular marker.