Figure 2

Apoptosis and necrosis of bMECs analyzed by flow cytometry with annexin V/propidium iodide (PI) dual staining. A Two dimensional scatter plots of FITC annexin V vs. PI from flow cytometry. Cells staining negative for FITC annexin V and PI in the lower left quadrant are live cells. Cells staining positive for FITC annexin V and negative for PI in the lower right quadrant represent early apoptosis. Cells staining positive for both FITC annexin V and PI in the upper right quadrant are the late apoptotic/necrotic cells. B Percentage of early apoptotic cells and late apoptotic/necrotic cells. Data are presented as mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 as compared with the control group.