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Table 5 Comparison of stimulation indices (SI) in parental and early and late proliferated CD4+; CD8+; pT+ and IgM+ carboxyfluorescein succinimidyl ester (CFSE) labelled splenocytes in control uninfected rabbits (con) and rabbits 2, 4, 6, 8 weeks afterE. cuniculioral infection.

From: Characterization of humoral and cell-mediated immunity in rabbits orally infected with Encephalitozoon cuniculi

 

CD4+ cells (SI)

CD8+ cells (SI)

pT+ cells (SI)

IgM+ cells (SI)

Week pi

Parental population

Proliferating

Parental population

Proliferating

Parental Population

Proliferating

Parental Population

Proliferating

Early

Late

Early

Late

Early

Late

Early

Late

con

1.1

1.2

1.8

1.0

1.3

1.2

0.8

1.0

1.7

1.0

1.3

1.6

2

21.9

1351.6*

7450.2*

2.7

65.9*

59.3*

2.0

66.5*

105.6*

3.1

16.6*

6.1

4

1.4

10.1

92.2**

2.8*

7.6*

107.1**

2.2

5.1*

64.3**

1.5

4.7

7.0

6

0.7

7.8*

29.8*

1.2

8.3*

96.3*

0.6

5.5**

33.3**

1.4

3.4

2.3

8

1.2

36.3**

712.4**

2.1

24.2**

1016.7**

0.9

23.2**

695.2**

3.0*

44.0**

15.3**

  1. Data are presented as mean of SI from 5 animals in each group. For tracking of cell proliferation, CFSE labelled cells were incubated with E. cuniculi spores for 6 days and then stained with monoclonal antibodies against rabbit lymphocytes. The absolute numbers of respective subpopulations of lymphocytes for each rabbit were determined by detection of CFSE intensity for parental, early proliferating and late proliferating cells and the absolute values of antigen-stimulated splenocytes of respective lymphocyte subpopulations were divided by the value of the same cell subpopulation from the control medium in respective stages in order to obtain a SI. The parameters with significant difference (P < 0.05) and (P < 0.01) when compare to control (con) uninfected rabbits are marked with * and ** respectively.