Characterization of humoral and cell-mediated immunity in rabbits orally infected with Encephalitozoon cuniculi

Table 2 **Antibody panel used for immunostaining of CFSE-labelled splenocytes after specific stimulation withE. cuniculispores or cultivation in cell culture medium only.**

No. of tube	CD molecule	Isotype	Clone	Source	Secondary antibody
1	CD8	IgG2a	IS27A	VMRD Inc., USA	R-PE
	CD4	IgG1	RTH1A	VMRD Inc., USA	AF 647
2	pT	IgG1	RTH21A	VMRD Inc., USA	R-PE
3	IgM	IgG1	MCA812G	Serotec	AF 647
4	CD14	IgG1	CAM36A	VMRD Inc., USA	AF 647
	CD45	IgG2a	ISC18A	VMRD Inc., USA	R-PE

Goat anti-mouse conjugates of appropriate subisotypes (Southern Biotechnology Assoc. Inc., Birmingham, USA) were used as secondary antibodies. CFSE-labelled cells without any primary or secondary antibodies and with appropriate isotype-matched controls were added to separate tubes for each antibody used and included in flow cytometry analysis.

ISSN: 1297-9716