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Table 1 Antibody panel used for immunostaining of peripheral blood and lymphoid organ cell suspensions for flow cytometry analysis in a rabbit after oral infection withEncephalitozoon cuniculi.

From: Characterization of humoral and cell-mediated immunity in rabbits orally infected with Encephalitozoon cuniculi

No. of tube

CD molecule

Isotype

Clone

Source

Secondary antibody

1

pT

IgG1

RTH21A

VMRD Inc., USA

R-PE

 

CD4

IgG2a

MCA799G

Serotec

AF 647

 

CD8-FITC

IgG1

MCA1576F

Serotec

–

2

IgM

IgG1

MCA812G

Serotec

R-PE

3

CD14

IgG1

CAM36A

VMRD Inc., USA

FITC

 

CD45

IgG2a

ISC18A

VMRD Inc., USA

R-PE

4

CD79α-PE

IgG1

HM57

DakoCytomation, DK

–

  1. Goat anti-mouse conjugates of appropriate subisotypes (Southern Biotechnology Assoc. Inc., Birmingham, USA) were used as secondary antibodies. Appropriate isotype-matched controls were added to separate tubes for each antibody used.