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Table 1 Antibody panel used for immunostaining of peripheral blood and lymphoid organ cell suspensions for flow cytometry analysis in a rabbit after oral infection withEncephalitozoon cuniculi.

From: Characterization of humoral and cell-mediated immunity in rabbits orally infected with Encephalitozoon cuniculi

No. of tube CD molecule Isotype Clone Source Secondary antibody
1 pT IgG1 RTH21A VMRD Inc., USA R-PE
  CD4 IgG2a MCA799G Serotec AF 647
  CD8-FITC IgG1 MCA1576F Serotec
2 IgM IgG1 MCA812G Serotec R-PE
3 CD14 IgG1 CAM36A VMRD Inc., USA FITC
  CD45 IgG2a ISC18A VMRD Inc., USA R-PE
4 CD79α-PE IgG1 HM57 DakoCytomation, DK
  1. Goat anti-mouse conjugates of appropriate subisotypes (Southern Biotechnology Assoc. Inc., Birmingham, USA) were used as secondary antibodies. Appropriate isotype-matched controls were added to separate tubes for each antibody used.