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Figure 2 | Veterinary Research

Figure 2

From: Characterization of humoral and cell-mediated immunity in rabbits orally infected with Encephalitozoon cuniculi

Figure 2

Representative flow cytometry analysis of CFSE labelled splenocytes in control uninfected rabbit (con) and rabbits in 2, 4, 6 and 8 weeks afterE. cuniculioral infection. Splenocytes were stimulated with E. cuniculi spores and after 6 days of incubation stained with specific monoclonal antibodies against cell surface markers. Relative numbers of parental (red) and early and late proliferating cells (green) for each lymphocyte subpopulation were determined in all animals and then, using the BD TruCount Tubes (BD Biosciences, Franklin Lakes, NJ, USA), recalculated to absolute numbers in order to obtained stimulation indices (SI).

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