Far-western blotting of rTsAP binding with IEC proteins. A SDS-PAGE analysis of IEC (lane 1) and C2C12 (lane 2) lysates. B Specific binding between rTsAP and IEC proteins. The membrane with the IEC proteins (lanes 1–6) was incubated by rTsAP (lanes 1–3) or BSA (lanes 4–6). Specific binding bands of rTsAP with IEC were identified by anti-rTsAP serum (lane 1) and infection serum (lane 2), but not by preimmune serum (lane 3). No binding of BSA with IEC were detected by anti-rTsAP serum (lane 4), infection serum (lane 5) and preimmune serum (lane 6). No binding of rTsAP with C2C12 was observed using anti-rTsAP serum (lane 7), infection serum (lane 8) and preimmune serum (lane 9).