Recognition and immunolocalization of TsAP in cross-sections of diverseT. spiralisstages by IFT using anti-rTsAP serum. The worm sections were probed with anti-rTsAP serum, and immunostaining was identified at the cuticle of ML, IIL and AW, and female intrauterine embryos. But normal mouse sera did not identify any worm tissue components of the parasitic nematode. Scale-bar = 50 μm.