Figure 2
Identification of the rTsAP. A SDS-PAGE analysis of the rTsAP. Lane M: Protein marker; Lane 1: lysate of recombinant E. coli incorporating pQE-80L/TsAP prior to induction; Lane 2: lysate of recombinant E. coli incorporating pQE-80L/TsAP following induction; Lane 3: the purified rTsAP. B Western blot analysis of the rTsAP. The lysates of pQE-80L/TsAP prior to induction (lanes 1, 4, 7 and 10) were not recognized by infection serum (lane 1), anti-rTsAP serum (lane 4), normal serum (lane 7) and anti-his McAb (lane 10). The lysates of pQE-80L/TsAP following induction (lanes 2, 5, 8 and 11) and rTsAP (lanes 3, 6, 9 and 12) were probed by infection serum (lanes 2 and 3), anti-rTsAP serum (lanes 5 and 6) and anti-his McAb (lanes 11 and 12), but not by normal serum (Lanes 8 and 9).