Figure 11

Suppression of siRNA 842 on TsAP activity, larval invasion, intestinal adult burden and fecundity, and muscle larval burden. A Suppression of siRNA 842 on TsAP enzymatic activity. The ML were transfected with 3 μM siRNA 842 and cultured for 3 days, the TsAP enzymatic activity in crude proteins of transfected ML to cleave the substrate Leu-pNA was assayed. B Suppression of siRNA 842 on larva intrusion of IEC. The siRNA 842-transfected ML were first activated into the IIL with swine bile for 2 h at 37 °C, subsequently the IIL larvae were inoculated onto the IEC monolayer and the intruded worms were examined under microscopy at 2 h after incubation. The results are expressed as the percent of penetrated larvae to all larvae utilized in each test and shown as mean ± SD of 3 independent tests. *P < 0.001 compared with PBS and control siRNA group. C Intestinal adult worm burden at 6 dpi. D Newborn larvae (NBL) production of adult females adults in 72 h. Worm burden (n = 10) and NBL production (n = 30) are shown as mean ± SD from siRNA 842, control siRNA and PBS group. E Muscle larvae per gram (LPG) of tissues at 60 dpi. *P < 0.001 relative to the PBS or control RNA group.