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Figure 6 | Veterinary Research

Figure 6

From: The role of O-polysaccharide chain and complement resistance of Escherichia coli in mammary virulence

Figure 6

Rough MPEC strains are better activators of inflammatory response in mammary epithelial cells. Using lentivirus vector, mammary epithelial cells EPH4 were transduced with luciferase gene under the control of 5 copies of the NF-kappa-B response element. Transduced EPH4 cells were treated with 1 µgr/mL LPS (blue line A), and the following E. coli strains at MOI = 1; P4 (black line A), P4∆GalE (green line A), P4∆GalU (red line A), P4-NR (black line B), P4-96 (blue line B), P4-NR∆GalE (green line B), P4-NR∆GalU (red line B). NF-kappa-B activation was measured via analysis of luciferase activity in the presence of 150 µg/mL d-luciferin (RLU; relative luminescence units) following infection with the above described bacterial strains using SpectraMax i3x multiple detection microplate reader (Molecular Devices, CA, USA). In all experiments, LPS and normal medium were used as positive and negative controls, respectively. Data points are mean for one experiment performed in triplicate and represent the results of the three similar experiments.

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