Comparison of immunohistochemically labelled T and B lymphocytes and macrophages in the placentas of animals carrying NVFs and VFs from the G-NcSpain7 group. Box-plot graphs representing the median number of cells, lower and upper quartiles (boxes) and minimum and maximum values (whiskers) of CD3 + (A), CD4 + (B), CD8 + (C), CD20 + (D), MAC387 + (E) and lysozyme + (F) cell populations in placentomes collected at 20 dpi with 107 tachyzoites of Nc-Spain7 at 110 days of gestation. Data from uninfected control placentomes at 20 dpi are also presented. ****, ***, ** and * indicate P < 0.0001, P < 0.001, P < 0.01 and P < 0.05 significant differences, respectively, and σ indicates P < 0.1. Three randomly selected medial placentomes from each animal were analysed.