Immunohistochemical labelling of parasite antigen in the placenta. N. caninum antigen immunohistochemically labelled in placentomes from the G-NcSpain7 group at 20 dpi. Brownish staining indicates a positive reaction. (A) Focal necrosis in the caruncular part of the placenta with labelled Nc-Spain7 antigens within trophoblast cells; HE, 10 × , bar 200 µm. (B) Labelled Nc-Spain7 antigens within trophoblast cells; HE, 40 × , bar 50 µm. Three randomly selected medial placentomes from each animal were analysed.