Comparison of the immunohistochemical labelling of ECM components. The distribution of fibronectin (A), vimentin (B) and collagen IV (C) is shown in placentomes from uninfected control animals (G-control) and Nc-Spain7- and Nc-Spain1H-infected animals at 10 and 20 dpi. Increased immunoreactivity of fibronectin was detected in Nc-Spain1H-infected placentomes at 10 dpi. At 20 dpi, a loss of fibronectin, vimentin and collagen IV staining was observed in the lytic areas of the Nc-Spain7-infected placentomes. Brownish staining indicates a positive reaction. For the magnification, see the bar in each individual image. Three randomly selected medial placentomes from each animal were analysed.