Table 1 Primer sequences used for quantitative PCR
Gene type | Forward primer (5′–3′) | Reverse primer (5′–3′) | Amplicon size (bp) | Gen Bank accession number |
|---|---|---|---|---|
ZJ1 M gene | ATGGACTCATCCAGGACAATCGGGCT | TTATTTCCTGAAAGGATTGTATTTAGCAATGG | 1095 | AF431744.3 |
ZJ1 M gene | TACTTTGATTCTGCCCTCCCTT | TAAGCAGAGCATTGCGGAAGA | 255 | AF431744.3 |
CXCLi2/IL-8 | CATCATGAAGCATTCCATCT | CTTCCAAGGGATCTTCATTT | 205 | NM_205498.1 |
CXCLi1 | CCGATGCCAGTGCATAGAG | CCTTGTCCAGAATTGCCTTG | 191 | NM_205018.1 |
TLR3 | ACAATGGCAGATTGTAGTCACCT | GCACAATCCTGGTTTCAGTTTAG | 189 | XM_025149682.1 |
TLR7 | TGTGATGTGGAAGCCTTTGA | ATTATCTTTGGGCCCCAGTC | 219 | XM_015273651.2 |
MDA-5 | GGACGACCACGATCTCTGTGT | CACCTGTCTGGTCTGCATGTTATC | 79 | NM_001193638.1 |
IFN-α | GGAGTTTTGAGGAGGGTGGG | CGCGTCTTCCTTCCTCCTTT | 175 | XM_004937092.3 |
IFN-β | AACACTGGATTGACCGCACA | GTCCCAGGTACAAGCACTGT | 200 | NM_001024836.1 |
IFN-γ | TGAGCCAGATTGTTTCGATG | CTTGGCCAGGTCCATGATA | 152 | NM_205149.1 |
Androgen receptor (AR) | AGTGCCAGCCCATCTTTCTC | CCTTTGCCCACTTGACGAC | 159 | NM_001040090.1Â |
LH receptor (LHR) | ACTCCTGCGCAAACCCATTC | CTCGGCTCTTACAGCAACCT | 99 | NM_204936.1 |
StAR | TTCAGCGAGATGGAGATGTCC | GGAACACCTTACCCACGTCC | 160 | NM_204686.2Â |
3b-hydroxysteroid dehydrogenase (3βHSD) | GGGCAAGACTGAGGTGAAAATC | TGTGTGGATGACGAGCGAG | 94 | XM_015294370.2 |
Gallus gallus hydroxysteroid 17-beta dehydrogenase 4 (17βHSD4) | CGCTGGAGGAGGTTTGGG | TGGGTACTGCTTTCCCTCCA | 167 | NM_204943.1 |
Cholesterol side-chain cleavage enzyme, P450scc | GTTGGGTGTCTACGAGAGCG | TTGCGGTAGTCACGGTATGC | 126 | NM_001001756 |
β-actin | GGTCATCACCATTGGCAATG | CCCAAGAAAGATGGCTGGAA | 66 | L08165 |
GAPDH | CCATCACAGCCACACAGAAGAC | TGGACGCTGGGATGATGTT | 93 | NM_204305 |