Figure 1

Photomicrograph of NDV induced histopathological changes in the testis of control and NDV infected white leghorn roosters. Normal progression of spermatogenesis (yellow arrows pointing various stages), with the formation of mature spermatids, few are attached with Sertoli cells, and small numbers of mature spermatids were present in the lumen of seminiferous tubules of control birds, at 3 and 5 dpi (A and C). Panel B illustrates the shortening of the adluminal compartment of seminiferous epithelium, decrease in number of Sertoli cells, individualized, shrunken spermatogonia with pyknotic nuclei (blue arrow heads), at 3 dpi. The inset shows individualised spermatogonia. Histology of testis at 5 dpi, denotes multifocal, and segmented, spermatogenic columns, necrotic cells (brown arrow heads), lipid vacuoles and proteinaceous homogenous material. Sertoli cells were rare and small numbers of spermatids were present in the lumen (D).