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Table 1 Criteria for determining the presence of the histological characteristics of the adventitial layer.

From: Fasciola hepatica coinfection modifies the morphological and immunological features of Echinococcus granulosus cysts in cattle

  Histological feature Criteria
Laminated layer disorganization Either whole laminated segments inside the adventitial layer or host cell infiltration between the laminated layer sheets At least one in the entire sample
Necrosis between the laminated and adventitial layer Presence of an eosinophilic zone devoid of cells without a fibrous structure Across one low-power magnification field (approximately 5 mm)
Calcifications Presence of strong haematoxylin staining without cells and with crystal structures At least one in the entire sample
Host cells in the germinal layer As previously described [42] At least one in the entire sample
Giant multinucleated cells Cells with 5 or more nuclei near the laminated layer At least one per high-power magnification field (1 mm)
Palisading macrophages The presence of foamy macrophages with cytoplasmic projections in contact with the laminated layer Across one low-power magnification field
Lymphoid follicles Circular structures with lymphocytes near the laminated layer At least one per low-power magnification field
Eosinophils Cells with eosinophilic cytoplasm near the laminated layer At least ten or more eosinophil nuclei per high-power magnification field
  1. When a feature was not recorded in both slides, it was considered absent.