Skip to main content
Figure 5 | Veterinary Research

Figure 5

From: Defining immune correlates during latent and active chlamydial infection in sheep

Figure 5

IL-10 production toC. abortusantigen. Peripheral blood mononuclear cells from the 67 sheep in the five experimental groups were purified from whole blood (as described in section “Blood collection and peripheral blood mononuclear cell (PBMC) preparation”) on five occasions and set up in lymphocyte stimulation assays in vitro with the UV-inactivated Chlamydia abortus (C. abortus) antigen (set up as described in section “Lymphocyte stimulation assays”). One set of the duplicate plates were harvested for culture supernatants after 96 h and analysed for interleukin (IL)-10 production (as described in section “Cytokine ELISAs”). The datasets from each experimental group are presented in individual line graphs (A–E). The data points are the arithmetic mean values for each cellular bleed and the error bars represent the standard error of the mean (SEM). The x axis represents the weeks post inoculation with C. abortus (intranasal inoculation (i/n) Groups 1–3 with i/n sham control Group 4; and sub-cutaneous inoculation (s/c) Group 5). The week numbering for groups 1–4 are consistent in relation to i/n whereas group 5 is in relation to s/c. The y axis represents the arithmetic mean IL-10 production in biological units/millilitre amounts. A Group 1 (low dose), B Group 2 (medium dose), C Group 3 (high dose), D Group 4 (sham control) and E Group 5 (sub-cutaneous control). The statistics summarised in the figure been derived from Linear Mixed Modelling (LMM) as described in detail in section “Statistical analyses”.

Back to article page