Table 1 Genes and primers used in this study
Names | Accession No. | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) | Product |
|---|---|---|---|---|
GAPDH | NM_204305.1 | GACGTGCAGCAGGAACACTA | ATGGCCACCACTTGGACTTT | 122 bp |
bcl-2 | NM_205339.2 | GAGTTCGGCGGCGTGATGTG | TTCAGGTACTCGGTCATCCAGGTG | 92 bp |
bax | XM_015290060.2 | CGCAAGGTCTACGCCATCATCTC | GCAGCAGACCAGCACCAAGTAG | 165 bp |
caspase-3 | NM_204725.1 | TACCGGACTGTCATCTCGTTCAGG | ACTGCTTCGCTTGCTGTGATCTTC | 166 bp |
NLRP3 | NM_001348947.1 | GCTCCTTGCGTGCTCTAAGACC | TTGTGCTTCCAGATGCCGTCAG | 150 bp |
caspase-1 | XM_025142104.1 | GTGCTGCCGTGGAGACAACATAG | AGGAGACAGTATCAGGCGTGGAAG | 179 bp |
IL-1β | NM_204524.1 | AGCAGCCTCAGCGAAGAGACC | GTCCACTGTGGTGTGCTCAGAATC | 90 bp |
IL-18 | NM_204608.1 | AGATGATGAGCTGGAATGCGATGC | ATCTGGACGAACCACAAGCAACTG | 97 bp |
IL-6 | NM_204628.1 | ATGGTGATAAATCCCGATGAAG | CCTCACGGTCTTCTCCATAAAC | 153 bp |
IL-8 | HM179639.1 | ACACTCCTAACCATGAACGGCAAG | CTGGCACCGCAGCTCATTCC | 114 bp |
TNF-α | AY765397.1 | CTCAGGACAGCCTATGCCAACAAG | GCCACCACACGACAGCCAAG | 178 bp |
IL-10 | NM_001004414.2 | CAGCACCAGTCATCAGCAGAGC | GCAGGTGAAGAAGCGGTGACAG | 94 bp |