Figure 7

DHM inhibited TLR4/NF-κB signalling pathway and inflammation in LPS-induced ileum injury. Original blots for TLR4, p65, p-p65 and GAPDH (A). Changes of TLR4 (B) protein expression levels, p-p65/p65 ratio (C), IL-6 (D), IL-8 (E), TNF-α (F) and IL-10 (G) mRNA expression levels in ileum after 60 mg/kg LPS exposure for 12 h followed by 14 days of 0.025%, 0.05% and 0.1% DHM treatment. Values are expressed as the mean ± SD for each group (n = 5). *p < 0.05 and **p < 0.01 represented all groups compred with thecontrol group. ^#p < 0.05 and ^##p < 0.01 represented all groups compared with the LPS group.