Splenic MRC1loMHCIIhicells were the main source of inflammatory cytokines. The mRNA expression of A IL-1β, B IL-6, C IL-12, and D iNOS in FACS-sorted MRC1loMHCIIhi and MRC1hiMHCIIlo cells from the spleen of chickens at 2 and 4 h post-LPS injection. Data are normalized according to the data of the PBS group (n = 10). Data are represented as the mean values ± SD. **P < 0.01, ***P < 0.001.