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Figure 2 | Veterinary Research

Figure 2

From: Characterization of splenic MRC1hiMHCIIlo and MRC1loMHCIIhi cells from the monocyte/macrophage lineage of White Leghorn chickens

Figure 2

Splenic MRC1hiMHCIIlocells exhibit phagocytic properties superior to those of MRC1loMHCIIhicells under steady-state conditions. For the in vitro phagocytosis assay, MRC1+ cells were sorted from splenocytes by positive selection. MRC1+ cells (1 × 106 cells/mL) were preincubated for 3 h and then incubated with A CTV-labelled dead cells (1 × 106 cells/mL) or B OVA-FITC (1 ng/mL) for 1 h at 39 °C or 4 °C. The gating strategy is shown in Additional file 3. Data obtained at 39 °C were normalized according to results obtained at 4 °C. The data represent A the percentages of the MRC1+ cell subsets in the CTV+ gate and B the mean fluorescence intensity (MFI) of OVA-FITC in MRC1loMHCIIhi and MRC1hiMHCIIlo cells (n = 10). Data are represented as the mean values ± SD. ***P < 0.001.

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