Table 1 Strains used in this study
From: Identification of a new effector-immunity pair of Aeromonas hydrophila type VI secretion system
Strain or plasmid | Description | Source |
|---|---|---|
Strain | ||
A. hydrophila NJ-35 | Wilde-type, isolated from diseased crucian carp, in China, Ampr | Laboratory stock |
A. hydrophila J-1 | Wilde-type, isolated from diseased crucian carp, in China, Ampr | Laboratory stock |
A. hydrophila NJ-3 | Wilde-type, isolated from pond water, in China | Laboratory stock |
A. hydrophilaATCC 7966 | Wilde-type, isolated from fishy milk, in USA | Laboratory stock |
A. salmonicida CS-2 | Wilde-type, isolated from pond water, in China | Laboratory stock |
A. media NJ-8 | Wilde-type, isolated from pond water, in China | Laboratory stock |
A. veronii XH-14 | Wilde-type, isolated from diseased Chinese bream, in China | Laboratory stock |
V. parahaemolyticus RIMD 2210633 | Wilde-type, isolated from a patient suffering from diarrhoea, in Japan | Laboratory stock |
E. coli SM10 | λpir+, Kanr | [28] |
E. coli BL21 | E. coli strain, competent invitrogen cells | CWBIO |
E. coli TOP10 | E. coli strain, competent invitrogen cells | CWBIO |
∆tle1AH | tle1AH gene deletion mutant from NJ-35 | This study |
ΔclpV | clpV gene deletion mutant from NJ-35 | [32] |
∆tle1-tli1tli2AH | tle1-tli1tli2AH gene deletion mutant from NJ-35 | This study |
C∆tle1AH | ∆tle1AH with the vector pMMB-tle1AH | This study |
C∆tli1AH | ∆tle1-tli1tli2AH with the vector pMMB-tli1AH | This study |
C∆tli2AH | ∆tle1-tli1tli2AH with the vector pMMB-tli2AH | This study |
C∆ tli1tli2AH | ∆ tle1-tli1tli2AH with the vector pMMB-tli1tli2AH | This study |
Plasmid | ||
pYAK1 | R6K-ori suicide vector, SacB+, Cmr | [27] |
pYAK1-tle1AH | pYAK1 carrying the flanking sequence of tle1AH, Cmr | This study |
pYAK1-tle1-tli1tli2AH | pYAK1 carrying the flanking sequence of tle1−tli1tli2AH, Cmr | This study |
pMMB207 | Low-copy-number vector, Cmr | [29] |
pMMB-tle1AH | pMMB207 carrying the tle1AH coding region, Cmr | This study |
pMMB-tli1AH | pMMB207 carrying the tli1AH coding region, Cmr | This study |
pMMB-tli2AH | pMMB207 carrying the tli2AH coding region, Cmr | This study |
pMMB-tli1tli2AH | pMMB207 carrying the tli1tli2AH coding region, Cmr | This study |
pMMB- tle1AH(6*His) | pMMB207 carrying the tle1AH+ 6*His coding region, Cmr | This study |
pMMB-Kan | pMMB207 carrying the Kan coding region, Cmr, Kanr | This study |
pMMB-Gen | pMMB207 carrying the Gen coding region, Cmr, Ger | This study |
pBAD/HisA | Expression vector, Ampr | Invitrogen |
pBAD-tle1AH | pBAD/HisA carrying tle1AH sequence | This study |
pBAD-peri-tle1AH | pBAD/HisA carrying peri + tle1AH sequence, peri is the PelB leader sequence | This study |
pBAD-peri-tle1AHS303A | pBAD/HisA carrying peri + tle1AHS303A sequence, peri is the PelB leader sequence | This study |
pGEX-4T-1 | Expression vector, Ampr | Invitrogen |
pGEX-tle1AH | pGEX-4T-1 carrying tle1AH sequence | This study |
pET-28a | Expression vector, Kanr | Invitrogen |
His-vgrG | pET-28a carrying vgrG sequence | This study |
His-hcp | pET-28a carrying hcp sequence | This study |