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Table 1 Strains used in this study

From: Identification of a new effector-immunity pair of Aeromonas hydrophila type VI secretion system

Strain or plasmid Description Source
Strain
A. hydrophila NJ-35 Wilde-type, isolated from diseased crucian carp, in China, Ampr Laboratory stock
A. hydrophila J-1 Wilde-type, isolated from diseased crucian carp, in China, Ampr Laboratory stock
A. hydrophila NJ-3 Wilde-type, isolated from pond water, in China Laboratory stock
A. hydrophilaATCC 7966 Wilde-type, isolated from fishy milk, in USA Laboratory stock
A. salmonicida CS-2 Wilde-type, isolated from pond water, in China Laboratory stock
A. media NJ-8 Wilde-type, isolated from pond water, in China Laboratory stock
A. veronii XH-14 Wilde-type, isolated from diseased Chinese bream, in China Laboratory stock
V. parahaemolyticus RIMD 2210633 Wilde-type, isolated from a patient suffering from diarrhoea, in Japan Laboratory stock
E. coli SM10 λpir+, Kanr [28]
E. coli BL21 E. coli strain, competent invitrogen cells CWBIO
E. coli TOP10 E. coli strain, competent invitrogen cells CWBIO
tle1AH tle1AH gene deletion mutant from NJ-35 This study
ΔclpV clpV gene deletion mutant from NJ-35 [32]
tle1-tli1tli2AH tle1-tli1tli2AH gene deletion mutant from NJ-35 This study
C∆tle1AH tle1AH with the vector pMMB-tle1AH This study
C∆tli1AH tle1-tli1tli2AH with the vector pMMB-tli1AH This study
C∆tli2AH tle1-tli1tli2AH with the vector pMMB-tli2AH This study
C∆ tli1tli2AH tle1-tli1tli2AH with the vector pMMB-tli1tli2AH This study
Plasmid
pYAK1 R6K-ori suicide vector, SacB+, Cmr [27]
pYAK1-tle1AH pYAK1 carrying the flanking sequence of tle1AH, Cmr This study
pYAK1-tle1-tli1tli2AH pYAK1 carrying the flanking sequence of tle1tli1tli2AH, Cmr This study
pMMB207 Low-copy-number vector, Cmr [29]
pMMB-tle1AH pMMB207 carrying the tle1AH coding region, Cmr This study
pMMB-tli1AH pMMB207 carrying the tli1AH coding region, Cmr This study
pMMB-tli2AH pMMB207 carrying the tli2AH coding region, Cmr This study
pMMB-tli1tli2AH pMMB207 carrying the tli1tli2AH coding region, Cmr This study
pMMB- tle1AH(6*His) pMMB207 carrying the tle1AH+ 6*His coding region, Cmr This study
pMMB-Kan pMMB207 carrying the Kan coding region, Cmr, Kanr This study
pMMB-Gen pMMB207 carrying the Gen coding region, Cmr, Ger This study
pBAD/HisA Expression vector, Ampr Invitrogen
pBAD-tle1AH pBAD/HisA carrying tle1AH sequence This study
pBAD-peri-tle1AH pBAD/HisA carrying peri + tle1AH sequence, peri is the PelB leader sequence This study
pBAD-peri-tle1AHS303A pBAD/HisA carrying peri + tle1AHS303A sequence, peri is the PelB leader sequence This study
pGEX-4T-1 Expression vector, Ampr Invitrogen
pGEX-tle1AH pGEX-4T-1 carrying tle1AH sequence This study
pET-28a Expression vector, Kanr Invitrogen
His-vgrG pET-28a carrying vgrG sequence This study
His-hcp pET-28a carrying hcp sequence This study