Figure 5
From: Identification of a new effector-immunity pair of Aeromonas hydrophila type VI secretion system
The interaction of Tle1AHwith VgrG was verified by an in vitro pull-down assay. Purified GST, GST-Tle1AH and His-VgrG were incubated with magnetic beads for the GST pull-down assay. Controls included the incubation with GST-Tle1AH alone, His-VgrG alone, or GST with His-VgrG. Bound proteins were washed off the beads with the elution buffer and detected by immunoblotting using anti-GST or anti-His antibodies.