Activity analysis of IFN-stimulated response element (ISRE) and gamma-activated sequence (GAS) elements after stimulation by chicken IFN. A–C Analysis of activation of ISRE and GAS elements induced by chicken type I, II, and III IFNs in DF1 cells. D Analysis of activation of ISRE and GAS elements induced by chicken type III IFN in LMH cells. Data are representative of three independent experiments. ns: not significant (P > 0.05), *P < 0.01, **P < 0.01, ***P < 0.001. Error bars indicate SEM.