Systematic identification of chicken type I, II and III ISGs. A Differentially expressed genes (DEGs) in chicken IFN-stimulated cells as detected by RNA-Seq. B Venn diagrams of up-regulated DEGs in DF1 and LMH cells stimulated with ChIFN-λ. Up-regulated genes induced by ChIFN-λ in both DF1 and LMH cells were considered as chicken type III IFN-stimulated genes (ISGs). C Statistics used for the identification of chicken type I, II, and III ISGs in this study.