Preparation of chicken types II and III interferon. A Chicken IFN-γ and IFN-λ (ChIFN-γ and ChIFN-λ) were prepared after expression in HEK293E cells. Purified ChIFN-γ and ChIFN-λ samples were subjected to SDS-PAGE. B The purified proteins were further verified by Western blotting using His antibody and ChIFN-γ and ChIFN-λ serum polyclonal antibody. C The bioactivity of the purified ChIFN-γ and ChIFN-λ proteins was detected in DF1 and LMH cell lines. The results are representative of three independent experiments.