Promotive effect of rTsATG4B protein and inhibitory effect of anti-rTsATG4B serum on larval invasion of the IEC monolayer in vitro. A Promotive effect of rTsATG4B on larval invasion. B The promotive effect on larval invasion was diminished when rTsATG4B was pre-incubated with E-64 (10 μM, 1:1); c The promotion rate of rTsATG4B protein on T. spiralis larval invasion of the IEC monolayer in vitro. Data were compared with the PBS group as the control. D The inhibition rate of anti-rTsATG4B serum showed a decreasing trend with increasing dilution and displayed a dose-dependent relationship with anti-rTsATG4B serum Data were compared with the PBS group as the control. *P < 0.01.