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Figure 5 | Veterinary Research

Figure 5

From: The cysteine protease ATG4B of Trichinella spiralis promotes larval invasion into the intestine of the host

Figure 5

Immunofluorescence assay of rTsATG4B binding to IECs (200×). IECs and C2C12 cells were pre-incubated with rTsATG4B at 37 °C for 2 h. Then, they were probed with different antibodies (anti-TsATG4B polyclonal antibody, T. spiralis-infected mouse serum and pre-immune mouse serum) and the FITC-conjugated goat anti-mouse IgG secondary antibody and were then counterstained with propidium iodide (PI; the fluorescent dye PI is a nuclear staining reagent that can stain the nucleus through the disrupted cell membrane and emits red fluorescence after embedding in double-stranded DNA).

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