Figure 4

Identification and expression of TsATG4B in the various developmental stages by immunolocalization. A–H Intact worms were recognized by anti-rTsATG4B mouse serum, as shown by IFA. Bright immunostaining was displayed on the surface of ML A, IIL B, 3 days female AW C, 3 days male AW D, 6 days female AW E, 6 days male AW F, and NBL G. ML H were probed with infection serum as a positive control. i–l Paraffin sections of Trichinella spiralis were recognized by anti-rTsATG4B mouse serum, as shown by IFA. Bright immunostaining was displayed on the cuticles and stichosomes of ML I and IIL J and in the embryos of 3 days female AW K. Paraffin sections of Trichinella spiralis ML L probed with infection serum were used as a positive control. Scale bars: 50 μm.