Table 1 Primer sequences used for real-time quantitative PCR
Target gene | Primer sequence | Product size (bp) |
|---|---|---|
β-actin | F: CAACACAGTGCTGTCTGGTGGTA R: ATCGTACTCCTGCTTGCTGATCC | 205 |
BAFF | F: CACGTCATCCAGCAGAAGGAT R: ACAAGAGGACAGGAGCATTGC | 120 |
BAFF-R | F: CCTGGCCCCACCATAAGG R: CATTACAGTCTCTCCTCACCCATACA | 120 |
CD40 | F: TGCACACCCTGTGAGAATGGT R: CGTTGCGTTTCCCTGTCTCTT | 120 |
CD40L | F: TGAAGTGGATGACGACGAGCTA R: TGGTGCAGAAGCTGACTTGTG | 120 |
TACI | F: GGCTCCTCATCCCAGTTCCT R: TTGTGCGTGAAGAAAGCTCTGT | 120 |
IL-1β | F: GCTCTACATGTCGTGTGTGATGAG R: TGTCGATGTCCCGCATGA | 80 |
IL-4 | F: AACATGCGTCAGCTCCTGAAT R: TCTGCTAGGAACTTCTCCATTGAA | 98 |
IL-6 | F: GCTCGCCGGCTTCGA R: GGTAGGTCTGAAAGGCGAACAG | 71 |
IL-15 | F: TAGGAAGCATGATGTACGGAACAT R: TTTTTGCTGTTGTGGAATTCAACT | 83 |
IL-17 | F: GCTGCAGCAAGAAAAGGAAGA R: GCCGTATCACCTTCCCATGT | 120 |
IFN-γ | F: AACCTTCCTGATGGCGTGAA R: GCTTTGCGCTGGATTCTCAA | 86 |