Figure 6

mRNA expression of cytokines in T cells in spleen from chickens vaccinated with inactivated H9N2 andB. subtilisspores. Chickens (N = 5) were administered H9N2 oil vaccine (commercially available), inactivated H9N2 or inactivated H9N2 and B. subtilis spores twice at one-week interval. Seven days after the last administration, splenocytes were re-stimulated with inactivated H9N2 for 24 h; then, T cells were isolated by magnetic bead sorting, and mRNA expression of A IFN-γ, B IL-17 and C IL-4 was examined by qRT-PCR. To determine the significance, one-way ANOVA followed by a Friedman test corrected by Dunn’s multiple comparison test was performed. Data are expressed as the mean values ± SDs. Different letters on each group denote a significant difference at P ≤ 0.05.