Figure 5

Comparison of the immune cell proportion after administration of H9N2 withB. subtilisspores or a commercial vaccine. Seven-day-old chickens (N = 5) were immunized twice with inactivated H9N2 and/or B. subtilis spores at one-week intervals. At one week post second immunization, single cells from splenocytes were stained with anti-KUL01, anti-Bu-1, anti-CD4 and anti-CD8 antibodies, and flow cytometry analysis was performed. The percentages and absolute numbers of A KUL01⁺ monocytes/macrophage cells, B Bu-1⁺ B cells, and C CD4⁺ and D CD8⁺ T cells were examined. To determine the significance, one-way ANOVA followed by a Friedman test corrected by Dunn’s multiple comparison test was performed. Data are expressed as the mean values ± SDs. Different letters on each group denote a significant difference at P ≤ 0.05.