Figure 4

CD4⁺and CD8⁺T cell proliferation after TCR stimulation co-treatment with the supernatant from splenocytes or monocytes/macrophages stimulated with inactivated H9N2 andB. subtilisspores. A CTV-labelled purified CD3⁺ T cells were cultured in anti-CD3 and anti-CD28 antibody-coated plates with supernatants from splenocytes or monocytes/macrophages stimulated with inactivated H9N2 and/or B. subtilis and analysed by using flow cytometry. B–E The proliferative populations of CD4⁺ T cells (B and C) and CD8⁺ T cells (D and E) were measured by CTV histograms and division index scores. To determine the significance, one-way ANOVA followed by a Friedman test corrected by Dunn’s multiple comparison test was performed. Data are expressed as the mean values ± SDs. Different letters on each group denote a significant difference at P ≤ 0.05.