Figure 2

Analysis of splenic B cells and innate immune cells in chickens administered inactivated H9N2 andB. subtilisspores. Seven-day-old chickens (N = 10) were immunized twice with inactivated H9N2 and/or B. subtilis spores at one-week intervals. Single cells from splenocytes were stained with the proper combination of anti-chicken CD3, Bu-1, and KUL01 antibodies, and flow cytometric analysis was performed. A Total splenocytes, B the percentage and C absolute number of KUL01⁺ monocytes/macrophages cells, and D the percentage and E absolute number of Bu-1⁺ cells were examined by using flow cytometry. To determine the significance, one-way ANOVA followed by a Friedman test corrected by Dunn’s multiple comparison test was performed. Data are expressed as the mean values ± SDs. Different letters on each group denote a significant difference at P ≤ 0.05.