Figure 3

Fine mapping of the epitopes recognized by the MAbs. A Schematic representation of VP1 antigenic regions (B–F). B Antigenic region E, which is further divided into E5′HVR, Econsv and E3′HVR regions. C Set of overlapping synthetic peptides encompassing truncated sequences within E5′HVR region. Based on the epitope mapping and neutralizing activity analyses performed, the 26 MAbs reported in this study were classified into five groups, G1-G5 (blue boxes). The MAbs representative of each group, shown in Figure 2, are depicted in black in this figure. Amino acids highlighted in red in E5′HVR sequence indicate previously described MAb escape mutations at positions 441, 448, 449 and 455 [15].