Table 1 Primers used in this study
From: Porcine DNAJB6 promotes PCV2 replication via enhancing the formation of autophagy in host cells
Primers | Sequences (5′–3′) | Vectors |
|---|---|---|
DNAJB6-F | CGGAATTCATGGTAGATTACTATGAAG | pGEX-4T-1 |
DNAJB6-R | CCGCTCGAGCTAGGGGCGGCCCTTGGTCGA | |
DNAJB61-99-F | CGGAATTCATGGTAGATTACTATGAAG | pGEX-4T-1 |
DNAJB61-99-R | GCGTCGACCTATTCCCTGGAGACGTCATC | |
DNAJB673-181-F | CGGAATTCGCCACCATGGGATTGAACGGTGGCAGTG | pGEX-4T-1 |
DNAJB673-181-R | GCGTCGACCTAACCAAACGCCGTGGAAGAGAAG | |
DNAJB6100-327-F | CGGAATTCGCCACCATGAGGGAATTTTTTGGTGGAAG | pGEX-4T-1 |
DNAJB6100-327-R | CCGCTCGAGCTAGGGGCGGCCCTTGGTCGA | |
F-DNAJB6-F | CGGAATTCATGGATTACAAGGATGACGACGATAAGGGAGG CATGGTAGATTACTATGAAG | pCI-neo |
F-DNAJB6-R | GCTCTAGACTAGGGGCGGCCCTTGGTCGA | |
P-DNAJB61-99-F | CGGAATTCATGGATTACAAGGATGACGACGATAAGGGA GGCATGGTAGATTACTATGAAG | pCI-neo |
P-DNAJB61-99-R | GCTCTAGACTATTCCCTGGAGACGTCATC | |
P-DNAJB673-181-F | CGGAATTCATGGATTACAAGGATGACGACGATAAGG | pCI-neo |
P-DNAJB673-181-R | GCTCTAGACTAACCAAACGCCGTGGAAGAGAAG | |
P-DNAJB6100-327-F | CGGAATTCATGGATTACAAGGATGACGACGATAAGGGAGGCATGAGGGAATTTTTTGATGG | pCI-neo |
P-DNAJB6100-327-R | GCTCTAGACTAGGGGCGGCCCTTGGTCGA | |
Cap-F | CCGCTCGAGATGACGTATCCAAGGAGGCGT | pEGFP-N1 |
Cap-R | CCCAAGCTTAGGGTTAAGTGGGGGGTC | |
Cap1-161-F | CCGCTCGAGATGACGTATCCAAGGAGGCGT | pEGFP-N1 |
Cap1-161-R | CCCAAGCTTAAGTACCGGGTGTGGTAGGAG | |
Cap118-234-F | CCGCTCGAGGTTGGATCCAGTGCTGTTATTC | pEGFP-N1 |
Cap118-234-R | CCCAAGCTTAGGGTTAAGTGGGGGGTC | |
Cap162-234-F | CCGCTCGAGCCCAAACCTGTCCTTGATTCC | pEGFP-N1 |
Cap162-234-R | CCCAAGCTTAGGGTTAAGTGGGGGGTC | |
Cap-F | CCGCTCGAGATGACGTATCCAAGGAGGCGT | pCI-neo |
Cap-R | CCCGTCGACAGGGTTAAGTGGGGGGTC |