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Table 2 Comparison of PUCV and HYV neutralisation titres for control and selected bovine field sera.

From: Hayes Yard virus: a novel ephemerovirus isolated from a bull with severe clinical signs of bovine ephemeral fever is most closely related to Puchong virus

Serum

Description

Neutralisation titre

PUCV

HYV

a-PUCV

PUCV immune mouse ascites fluid (IMAF)

1/2560

1/640

a-BEFV

Bovine serum—vaccinated against BEFV

–a

–

164 066

Bovine serum—ELISA-positive to KIMV

ELISA-negative to PUCV

–

–

163 862

Bovine serum—weak ELISA-positive to PUCV

–

–

164 934

Bovine serum—strong ELISA-positive to PUCV

1/20

1/160

158 733

Bovine serum—ELISA-positive to PUCV (recent seroconversion)

–

1/20

  1. aA titre of < 1/20 was considered negative.