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Table 1 Primers used in qRT-PCR

From: Mycoplasma gallisepticum triggers immune damage in the chicken thymus by activating the TLR-2/MyD88/NF-κB signaling pathway and NLRP3 inflammasome

S. No.Gene namePrimers (from 5′ to 3′)Product Length
1TLR2Forward 5′-TCGCTCCAACACCTTCGCATTC-3′181
Reverse 5′-GATTGTCACCGTCGATCCTCAGC-3′
2NF-κBForward 5′-CACATGGTGGTGACCGCCAATAG-3′194
Reverse 5′-GTGCCATCGTATGTAGTGCTGTCC-3′
3Caspase-1Forward 5′- GTGCTGCCGTGGAGACAACATAG-3′179
Reverse 5′- AGGAGACAGTATCAGGCGTGGAAG-3′
4NLRP3Forward 5′- GCTCCTTGCGTGCTCTAAGACC-3′150
Reverse 5′- TTGTGCTTCCAGATGCCGTCAG-3′
5IL-10Forward 5′- CAGCACCAGTCATCAGCAGAGC-3′94
Reverse 5′- GCAGGTGAAGAAGCGGTGACAG-3′
6IL-18Forward 5′- AGATGATGAGCTGGAATGCGATGC-3′97
Reverse 5′- ATCTGGACGAACCACAAGCAACTG-3′
7PFKForward 5′-GTGAGAGTTGGCATAACGGAAGGC-3′191
Reverse 5′-CGCATCTGGTCAGCAATCTTCTCC-3′
8PKForward 5′-CTCAGCCAACTCTCCGTGATATGC-3′175
Reverse 5′-TCCACTGCTTCCAAGAACGATGAC-3′
9SDHBForward 5′-TGGACGGACTCTATGAGTGCATCC-3′167
Reverse 5′-TTGAAGTTGTGCCAGGCGTTCC-3′
10LDHBForward 5′-GCAGGTGTTCGTCAGCAAGAGG-3′176
Reverse 5′-GGCAGGCCACTCAACTTCCATG-3′
11LDHAForward 5′-TGCCTGTCTGGAGCGGAGTG-3′116
Reverse 5′-GTCCACCACCTGCTTGTGAACC-3′
12HK1Forward 5′-TCATGGCTGTTGTGAACGATACCG-3′132
Reverse 5′-GGTCAATGTGCCGCATCTCCTC-3′
13HK2Forward 5′-TGGAGGTGAAGCGGAGGATGAG-3′177
Reverse 5′-GCACCAGCAGCACACGGAAG-3′
14ACO2Forward 5′-CCTGTGGACAAGCTGAGCATCG-3′129
Reverse 5′-CTGCGACTCGTTGAAGGTGTGG-3′
15MYD88Forward 5′-AAGGTGTCGGAGGATGGTGGTC-3′
Reverse 5′-GGAATCAGCCGCTTGAGACGAG-3′
120
16Beclin-1Forward 5′-ACCGCAAGATTGTGGCTGAAGAC-3′163
Reverse 5′- TGAGCATAACGCATCTGGTTCTCC-3′
17mTORForward 5′-AACCACTGCTCGCCACAATGC-3′120
Reverse 5′-CATAGGATCGCCACACGGATTAGC-3′
18TNF-αForward 5′-TGATCGTGACACGTCTCTGC-3′88
Reverse 5′- CAACCAGCTATGCACCCCAG-3′
19IL-6Forward 5′-TTCACCGTGTGCGAGAACAGC-3′80
Reverse 5′- CAGCCGTCCTCCTCCGTCAC-3′
20IL-1βForward 5′-AGCAGCCTCAGCGAAGAGACC-3′90
Reverse 5′-GTCCACTGTGGTGTGCTCAGAATC-3′
21ULK-1Forward 5′-AATCACAGACTCTGCTGGGC-3′166
Reverse 5′-AGTGTCCGCATAGTGTGAAGG-3′
22ATG5Forward 5′- GGACGCATACCAACCTGCTT-3′200
Reverse 5′-TGCCATTTCAGTGGCGTACC-3′
23DyneinForward 5′-CGTTGCCAGCGTTACACCTATCC-3′163
Reverse 5′- GCCAGGACTGCCACCAACAC-3′
24β-actinForward 5′-CAACACAGTGCTGTCTGGTGGTAC-3′199
Reverse 5′-CTCCTGCTTGCTGATCCACATCTG-3′